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sirA [2017-10-03 11:26:08]
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sirA [2017-10-03 11:26:08]

sporulation protein, inhibits DNA replication initiation in cells committed to sporulation
locus
BSU17900
pI
7.35
mw
18.00 kDa
protein length
148 aa Sequence Blast
gene length
444 bp Sequence Blast
function
control of chromosome copy number
product
inhibitor of DNA replication
essential
no
synonyms
yneE,yoxF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]

    • Gene

    Coordinates
    1,922,017 → 1,922,463

    Phenotypes of a mutant

  • over-replication during sporulation [Pubmed|19682252]

    • The protein

    Catalyzed reaction/ biological activity

  • interacts with [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] and displaces it from the replication origin, resulting in inhibition of replication [Pubmed|19682252]

    • Structure

  • [PDB|4TPS] (in complex with N-terminal domain of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]) [Pubmed|25041308]

    • [SW|Localization]

  • accumulates at the [SW|replisome] in sporulating cells (perhaps in [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]-dependent manner) [Pubmed|25041308]

    • Expression and Regulation

    Operons

    genes
    [gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]-[gene|9C151A07F22920F58D635B82545DA76C7087AA8E|yneF]
    description
    [Pubmed|9068642]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|14651647,19329632], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647,19329632]
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    Biological materials

    Mutant

  • MGNA-B389 (yneE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1388 NBRP B. subtilis, Japan]
  • BKE17900 (Δ[gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC, downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA
  • BKK17900 (Δ[gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC, downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA

    • References

    Reviews

  • 20157337,22933559,28075389

    • Original publications

  • 19682252,21239581,14651647,19329632,9068642,25041308,27489185